logotype

Instrumentation

CSGI possesses state-of-the-art instrumentation in the field of reference. Instruments are available as a service to external users at moderate cost and to the Universities belonging to CSGI network. A list of CSGI instruments is reported below.

 

Florence

  • TA Instruments Discovery Hybrid Rheometer HR3. This rheometer is at controlled shear stress working in the torque range 0.5 nN.m - 200 mN.m. It is possible to perform all the rheological standard tests to make a complete mechanical characterization of the material to be studied. The behavior of dynamic viscosity, viscoelastic functions (G’ and G” and complex viscosity), creep, and yield stress of materials can be easily performed. The system is equipped with a peltier plate that allow to explore the rheological properties of fluids in a T range between -40 °C and 200 °C. The available geometries are are a smooth plate (diameter 40 mm) and a crosshatched plate (diameter 40 mm). The system is further equipped with a Dynamic Mechanical Analysis system (tension set up) that can provide a direct measure of the Young Modulus and with an Environmental Test Chamber that allows the use of the DMA set up in the temperature range of -160°C to 600°C.
  • Laser Scanning Confocal Microscope Leica TCS SP2 equipped with a ISS FCS module, with 4 lasers, providing eight available laser lines. The confocal microscope is equipped with three spectral detectors for fluorescence (PMT) suitable for imaging (and z-stack), spatially resolved emission,  spatially resolved FRET, FRAP and a transmission PMT. The FCS module is equipped with two APD, suitable for FCS and FCCS experiments.
  • Laser Scanning Confocal Microscope Leica TCS SP8 with a DMI8 microscope with a Picoquant FCS module, with 4 lasers, providing eight available laser lines. It is equipped with one external PMT for transmission and five internal spectral detectors two PMT, one Hybrid and two cooled Hybrid detectors for Single Molecule Detection, suitable for imaging (and z-stack), spatially resolved emission,  spatially resolved FRET, FRAP, FCS and FCCS. The microscope table is motorised both in z and in xy, allowing the mapping of extended samples.
  • Zeiss Sigma FE-SEM. The SEM is equipped with the GEMINI column with its in-lens secondary electron detection, and grants unparalleled resolution, contrast and brightness for imaging highly topographical samples. High-resolution images can be obtained both on conducting and non-conducting samples.
  • EDAX: Oxford Instruments INCA X-act. Energy dispersive x-ray spectrometry. The x-act is a fully quantitative SDD with excellent performance at low and high count rates. Detects elements from Be to Pu.
  • Agilent Cary 670 FTIR and Cary 620 Microscope FTIR Configurable (with GeATR). Equipped with three detectors (FPA 128x128 for imaging, MCT, DTGS), NIR and MIR sources.
  • TA-Instruments Q-1000. Study of thermal events involving heat transfer or heat capacity changes (phase transitions, chemical reactions) with determination of enthalpies and kinetics of the processes (rate constants, activation energies, kinetic laws). Fields of application: dehydration reactions, decomposition of organic materials, phase changes, cement hydration, quantitative determination of inorganic hydrated salts as gypsum, hydrated oxalates, nitrates; phase transition in liquid crystals, vesicles and liposomes. Range of temperature: – 90 °C - 550 °C.
  • Perkin Elmer DSC-7. Study of thermal events involving heat transfer or heat capacity changes (phase transitions, chemical reactions) with determination of enthalpies and kinetics of the processes (rate constants, activation energies, kinetic laws). Fields of application: dehydration reactions, decomposition of organic materials, phase changes, cement hydration, quantitative determination of inorganic hydrated salts as gypsum, hydrated oxalates, nitrates; phase transition in liquid crystals, vesicles and liposomes. Range of temperature: – 180 °C - 550 °C.
  • TA Instruments Q-600 (DTA-TG). The instrument allows the simultaneous determination of thermal events and gravimetric changes in the temperature range room temperature-1500 °C. Fields of application: all the phenomena where thermodynamic transition and/or chemical processes occur involving enthalpy and/or weight changes.
  • GC: A KSV instrument
  • Brewster Angle: NFT, Germany EP3. Brewster Angle Microscope with Ellipsometry Imaging (BAM). A unique combination of ultra high contrast microscopy and ellipsometry: a novel analysis tool for film thickness and surface morphology characterization. Applications span from the study of domains and order phenomena in monolayers at liquid-air interface to characterization of ultrathin films on solid supports (silicon, quartz, gold,).
  • EDAX: Oxford Instruments INCA X-Sight. Energy dispersive x-ray spectrometry. EDS microanalysis system, in addition to the conventional x-ray analysis capabilities, produces high-resolution digital elemental distribution maps. INCA Energy software makes reliable element identification easy with accurate AutoID and spectrum overlay for investigating peak overlaps. Point & ID for image centric analysis for analysing materials where spatial information is important. Cameo+ program shows you what the sample would look like if your eyes could see X-rays, enabling phase distribution to be quickly and reproducibly determined.
  • Rudolph Research (USA) 43702-200E. Null Ellipsometer. Ellipsometry is a non-destructive optical method to characterize ultrathin films (0.1 nm). With our single wavelength (6328Å) nulling ellipsometer the thickness and refractive index of a dielectric film can be readily determined on a variety of reflecting substrates.
  • BioRad UMA-400. Allows to collect IR spectra in reflectance and trasmittance mode directly on a solid sample on regions from 250x250 µm up to 10x10 m.
  • Reichert Zetopan. It works in reflectance and transmission mode with polarizers and interferential contrast according to Nomarski with digital camera Nikon, model . Objectives 5.5x, 11x, 28x, 56x.
  • Wild M5A. It works in reflectance mode with high focal length allowing to easily manipulate the samples. Objectives 6x, 12x, 25x, 50x.
  • Leica Cambridge Stereoscan S360. The Scanning Electron Microscope (SEM) is one of the most versatile and widely used tools of modern science as it allows the study of both morphology and composition of all kind of materials. High-resolution images of the morphology of a specimen, with great depth of field, at very high magnifications can be obtained. Compositional analysis of a material may also be obtained by monitoring secondary X-rays produced by the electron-specimen interaction. Characterisation of fine particulate matter in terms of size, shape, and distribution may be performed. The sample preparation is relatively easy since SEM only require the sample to be conductive. Specimens must be coated with a very thin layer of conductive material. CSGI has a Agar auto gold sputter system and as Agar auto carbon (graphite) coater.
  • Densimetry: A KSV instrument
  • Paar Physica UDS-200. This rheometer is at controlled shear stress working in the torque range 0.5 ?N.m - 150 mN.m. It is possible to perform all the rheological standard tests to make a complete mechanical characterization of the material to be studied. The behavior of dynamic viscosity, viscoelastic functions (G’ and G” and complex viscosity), creep, and yield stress of materials can be easily performed. The viscosity range (between 0.5x10-3 and 8.8x108 Pa x s) is strictly related to the measuring system used (concentric cylinder geometry for low viscous systems as aqueous emulsions and cone-and-plate, parallel-plate geometries for higly viscous samples as gels). The temperature range is also related to the measuring system (-10/400°C for concentric cylinder geometry and –40/160°C for cone-and-plate, parallel-plate geometries).
  • Rigaku Nanoviewer is based on Confocal Max-Flux™ Optics (Osmic) coupled with a 2-D CCD detector (Mercury70) mounted on a MicroMax 007 rotating anode generator. The Nanoviewer SAXS spectrometer has a standard of about 600 Å resolution as maximal dimension, but can be reconfigured for different resolution due to its modular design (from 0.6 up to 2 m fully evacuated flight path).
  • Hecus X-Ray System GmbH Kratky camera coupled with two 1-D Position-Sensitive Detectors (Methane/Ar) mounted on a ID3003 generator (Seifert). The Q-range accessed by the SWAXS system goes from 0.05 - 6 1/Å (corresponding to Bragg's spacings of 120-1 nm) while the wide angle region simultaneously investigated span from 18 to 26 degrees (corresponding to Bragg's spacings of 0.34 - 0.49 nm).
  • Laser Granulometry: A KSV instrument
  • Jasco J-715. Circular Dichroism measures the difference in absorption coefficients of an optically active sample for left and right circularly polarized light. It is the method of choice for the determination of conformational details, such as protein secondary and tertiary structure, to monitor folding or binding and to assess purity of optically active substances (polymers, pahrmaceuticals, etc..). A circulating bath temperature control is available.
  • Perkin Elmer, Italy LS50B. Spectrofluorimeter. The spectrometer can be used in fluorescence and phosphorescence measurement modes. 3D excitation/emission scans as well as single and multiple wavelength kinetics can be performed. Our instrumentation includes automated accessories to address a wide range of bio and nano-research applications: a set of polarizers allows steady-state fluorescence anisotropy studies whereas the Front Surface accessory is ideal for analyzing films, papers, powders and other flat samples.
  • BioRad FTS-40. Qualitative and quantitative study of organic and inorganic molecules. The apparatus has DTGS and MCT detectors and the following accessories: Golden Gate Graseby-Specac, DRIFT (Diffuse Reflectance Infared Fourier Transform) Graseby-Specac, ATR (Attenuated Total Reflectance) with Germanium crystals.
  • Nicolet Nexus 870. Equipped with an external optical bench, two external detector modules (MCT and TRS), a Hind Photoelastic Modulator and a Continuµm infrared microscope.
  • Perkin Elmer, Italy, Lambda 900. Spectrophotometer. UV/Vis/NIR Spectrophotometer with depolarizer/polarizer accessories for acquisition of standard absorption spectra, kinetic measurements and linear dichroism experiments. The instrument is equipped with a software-driven temperature probe and a fiber-optics accessory for experiments on non-conventional samples (i.e. water-air interface, nanofilms on quartz slides).
  • (Rame’-Hart, USA) Advanced Automated Goniometers Model 300. Contact Angle Goniometer. Contact angle measurements are an important tool to characterize the wettability of solids. The instrument is fully software driven - allowing the user to take automated contact angle measurements as a function of time. The instrument is equipped with an Automated Dispenser tool and control pump for single drops for advanced/receding studies. This hydraulic system uses disposable tips for multiple liquid studies and quick changes from one specimen to another.
  • NIMA (England) 302MC Multiple compartment. Langmuir Film Balance. The trough is equipped with 2 compartments for transfer of monolayer between different subphases. This configuration is optimized for the investigation of protein/monolayer interactions and biomembrane and enzyme kinetic reaction studies.
  • NIMA (England) 702BAM Film Balance for Brewster angle microscopy. Langmuir Film Balance. Analytical film balance for use with the Brewster angle microscope. Allows simultaneous observation of monolayer morphology and surface pressure-area isotherms.
  • Lauda (Germany) Filmwaage II. Langmuir Film Balance. Standard continuous compression film balance with a single barrier equipped for the simultaneous measurements of surface pressure and surface potential data. All our instruments are equipped with a thermostatic bath for temperature control of the subphase.
  • KSV (Finland). KSV 5000. Langmuir-Blodgett Instruments. Langmuir-Blodgett instrument for unsupervised deposition of ordered multilayer LB-films. Two separate troughs allow the simultaneous preparation of two different monolayers for alternate LB film deposition.
  • KSV (Finland) KSV 3000. Langmuir-Blodgett Instruments. Langmuir film balance for conventional monolayer analyses and Langmuir-Film preparation. Barrier drives can be used for both single sided or symmetric compression depending on the rheology of the film-forming materials.
  • Coulter SA-3100 (SA-BET). The instrument allows the determination of specific areas (m2/g) in the range 1-600 m2/g and the simultaneous evaluation of the pore size distribution in the range 6-200 nm, by means of N2 adsorption isotherms. Fields of application: heterogeneous catalysis, fine powders, nanomaterials, adsorption.
  • KSV Sigma 70. Static Tensiometry. KSV Sigma 70 is a fully computer controlled and automatic tensiometer for the measurement of surface/interfacial tension (0.25 mN to 25 mN range) Standard programs include automatic determination of CMC, push and pull surface and interfacial tension measurements, podwer wettability and measurement of dynamic advancing and receding contact angles.
  • IT Concepts. The Tracker. Dynamic Surface Tensiometry. Surface or interfacial tension is measured using the pending drop method on a time span from 500 ms to even some hours. When equilibrium is reached the drop area can be periodically deformed to determine the viscoelastic properties of the interfaces, which are among the key parameters correlated to the stability of the emulsions and foams.
  • Freeze-Drying: A KSV instrument
  • Lipex Biomembranes. The extruder. Preparation of unilamellar monodisperse liposomes by extrusion under pressure (up to 35 atm) through polycarbonate filters (200, 100 or 100 nm). The extruder can be thermostated with an external circulating water bath. Liposomes are mimics of cell membranes and can be used to investigate structure and function of membrane proteins as well as durg-delivery vehicles.
  • Ecochemie Netherland Autolab PGSTAT30. Potentiostat/galvanostat. This is a potentiostat/galvanostat with a compliance voltage of 30V and a high bandwidth (>1MHz) making it especially suited for Electrochemical Impedance Spectroscopy in combination with the FRA2 modules. The PGSTA30 can perform all electrochemical techniques including cyclic voltammetry and amperometry.

Up

Bari

  • XPS spectrometer Theta Probe. “Theta Probe” Thermo VG Scientific Spectrometer for X-ray Photoelectron Spectroscopy (XPS), equipped with microspot and monochromatic X-ray source, non-monochromatic twin anode (Al/Mg) X-ray source, combined low-energy electron/Ion flood gun, Argon ion gun, Double-focusing full 180° spherical sector electron Analyzer, Multi-element electrostatic RADIAN input lens for spectroscopy.
  • XPS spectrometer VersaProbe II. “VersaProbe II” PHI Spectrometer, equipped with monochromatic (Al) micro-focused scanning X-ray Source, non-monochromatic twin anode (Al/Mg) X-ray source, Dual Beam Charge Neutralization system, Ar ion gun, 128 channel detector. Both instruments are dedicated to surface analysis and allow: to determine surface elemental composition; to identify and quantify the chemical environments on the material surface (speciation analysis); to operate in non-destructive Parallel Angle Resolved-XPS (PAR-XPS) mode for studying elemental and speciation distribution depth profiles without tilting (only Theta Probe); to map the surface in order to verify the in-plane distribution/homogeneity; to perform Large Area XPS (only VersaProbe II).
  • Transmission Electron Microscope FEI “Tecnai G2 Twin Spirit” equipped with live CCD camera.The microscope allows the bidimensional observation (and image aquisition) of either inorganic and biological samples with high spatial resolution in the nm-range.
  • Perkin Elmer “PinnAAcle AS900-Z” spectrometer for Electro-thermal atomic absorption spectroscopy (ETAAS) equipped with transversal heated graphite tube atomizer, auto-sampler (up to148 positions), mono- and multi-element lamps, and longitudinal Zeeman background correction. This instrument allows metal trace analysis depending on the lamps employed. The workstation can be employed for electrochemical characterizations/experiments as they allow performing several electrochemical techniques.
  • Princeton Applied Research “PARSTAT 4000” Potentiostat-Galvanostat (Compliance Voltage ±48 V, Compliance Current ±4 A (standard)) with additional low-current module (VersaSTAT-LC), accessories for Electrochemical Impedance Spectroscopy, rotating disc electrode.
  • CH Instruments “CHI1140b” Potentiostat-Galvanostat (Compliance Voltage ±25 V, Compliance Current ±2 A). The workstation can be employed for electrochemical characterizations/experiments as they allow performing several electrochemical techniques.
  • Dynamic Light Scattering and Zeta-potential set-up including: surface zeta-potential facility and microrheology facility.  Malvern Zetasizer Nano ZS equipped with Surface zeta potential accessory Research Software and Microrheology software.
  • Laser Scanning Confocal Microscope equipped with diode laser at 405 nm, argon laser (458nm, 476nm, 488nm, 514nm), pulsed white laser. The instrument (Leica) can acquire space resolved emission and excitation  spectra and is also equipped with Picoquant facilities for Fluorescence Correlation Spectroscopy (FCS) and Fluorescence-lifetime imaging microscopy.
  • NMR spectrometer 500 MHz (Agilent) equipped for NMR in solution and solid state and pulsed gradient units for DOSY
  • Photoelectron spectroscopy (XPS)
  • Multiparametric Surface Plasmon Resonance (SPR) apparatus (SPR Navi™ Bionavis)

Up

Bologna

  • Amersham-Pharmacia Gradi-Frac, UV1 monitor. Liquid chromatography for protein characterization.
  • Reichardt Biovar. Optical microscopy for microbial characterization
  • Stratagene Stratalinker, UV-VIS Spectrophotometry. For mutagenesis, DNA and RNA cross-link.
  • Perkin Elmer Lambda 9, UV-VIS Spectrophotometry. For protein assays, Kinetics.
  • Heto Dry Winner. Freeze drying system for the preparation of microbial lyophilised stocks.
  • Heto Gallenkamp. Incubator for microbial cultures.
  • Alessandrini 810. Incubator for microbial cultures
  • Hybaid PCR Sprint. Polymerase chain reaction.
  • Heto Vacuum Centrifuge. For vacuum concentration of nucleic acids.
  • Asalair 700. Preparation of PCR reactions.
  • Bio-Rad Gene Pulser II (TRANSF). Electroporation of microorganisms.
  • Amersham-Pharmacia Gene Quantification. Quantification of nucleic acids.
  • Bio-Rad GelDoc IQ (GEL-DOC). Molecular imaging of nucleic acids upon electrophoresis.

Up

Campobasso

  • Oxford Link INCA EDX Detector, Electron probe X-ray microanalysis techniques: use the characteristic X-rays generated from a sample bombarded with electrons to identify the elemental constituents comprising the sample.
  • Thermo Scientific Haake Mars III, rotational and dynamic rheometer specifically designed for quality control and research of fluids, slurries, pastes, gels, creams, suspensions, and polymer melts.
  • Malvern Nano-ZS90, Size measurement from 0.3nm (diameter) to 5 microns using 90 degree scattering optics.
  • Malvern Nano-ZS, Size measurement from 0.3nm (diameter) to 5 microns using using patented NIBS (Non-Invasive Back Scatter)Molecular weight measurement down to 980Da.
  • Varian, Cary Eclipse, Steady state fluorescence, phosphorescence and chemoluminescence measurement modes.
  • Thermo Scientific, ITQ Series GC-Ion Trap MS. GC-MS analysis for food and environmental safety.
  • Malvern, Nano ZS 90, Zeta potential measurement using laser Doppler electrophoresis (LDE); assess the charge stability of a disperse system, and assist in the formulation of stable products.

Up

Pavia

  • Sensys High-Pressure DSC – Setaram. Technical details: 25 °C – 800 °C at 1 bar; 25 °C – 600 °C at 130 bar (hydrogen gas) – for hydrogen sorption studies. The instrument can be coupled with the PCTPro-2000 apparatus for simultaneous calorimetric – manometric analysis.
  • Q2000 – TA Instruments. Technical details: -90 °C – 725 °C under inert atmosphere. Modulated DSC.
  • Q600 – TA Instrument. Simultaneous TGA-DSC. Technical details: 25 °C – 1500 °C under inert atmosphere.
  • Q5000IR – TA Instruments. Technical details: 25 °C – 1200 °C under inert atmosphere. Infrared radiation heating.
  • Hiden Isochema IGA-003 gravimetric analyser for study of gas - solid interactions at controlled pressure and temperature (from vacuum to 20 bar and from -20 °C to 500 °C).
  • Nikon Eclipse ME600 microscope equipped with Linkam high temperature stages (THMS600 and THMS600-PS) working from -196 °C up to 600 °C and 14 bar and with a JVC camera to take pictures and movies during observations.
  • Zeiss Evo-MA10 SEM with X-Max Oxford 50 mm2 EDS microanalysis. Cooling stage to work from -50 °C to + 60 °C for research in the pharmaceutical field. Home-made sample holder to transfer air-reactive samples from the glove-box to the SEM and vice versa. SEM
  • A Perkin Elmer apparatus for densimetry
  • Static LS
  • A Perkin Elmer apparatus for laser granulometry
  • Malvern Instruments Mastersiezer 2000. Technical details: it measures materials’ size from 0.02µm to 2000µm. Particle sizer
  • XRF
  • Thermo Scientific Nicolet iS10 FT-IR spectrometer working with transmittance, ATR and diffuse reflectance with resolution up to 0.5 cm-1
  • Termo Electron Corporation Sorptomatic 1990. Technical details: specific surface of the samples: from 0.2 m2/g (according to the sample nature, using nitrogen). Pore volume: from 0.0001 cm3/g BET
  • A Perkin Elmer apparatus for freeze drying
  • Gouy interferometry
  • Hardness testing
  • Hot Isostatic press
  • Laser Diffractometer
  • Mac-Zehnder interferometry
  • Potensiostat: Frequency Response Analyzer (FRA) Autolab PGSTAT30 apparatus operating in the frequency range 1-106 Hz.
  • Taylor dispersion
  • Bruker D5005 powder X-ray diffractometer with polythermal chamber under controlled atmosphere from  -170 °C up to 1100 °C; software for acquisition and elaboration of diffraction data with access to Powder Diffraction Patterns Data of organic, inorganic and mineral materials. Bruker air-tight sample holder for analyses of air-sensitive samples at room temperature and Silicon slide sample holder (low background) for samples in small amounts. Suitable software for ab initio structural investigation and structural refinement (Bruker TOPAS4.0).
  • PCTPro-2000 gas analyzer to study gas-sorption processes (in particular, hydrogen sorption) from vacuum to 200 bar at T ranging from 25 °C to 600 °C. The instrument can be coupled with the Sensys DSC for simultaneous calorimetric – manometric analysis.
  • Mixer Turbula (W. A. Bachofen) for powders mixing and homogenisation.
  • Fritsch Pulverisette 4, 6, 7 planetary mills (up to 1100 rpm) with alumina, agate, stainless steel, tungsten carbide, zirconia,vials and balls to work up to 130 bar with temperature and pressure monitoring.

Up

Siena - Chemistry

  • JASCO HPLC. High-performance liquid chromatography. HPLC JASCO assembled with: AS-2055 Plus, BS-997-01, PU-2089 Plus, MD-2010 Plus, HV-2080-01.
  • JASCO J-815. Circular dichroism. Field of applications: Protein folding and conformational studies, Enzyme kinetics.
  • Bruker Elexys E500 AR. Electron Paramagnetic Resonance.EPR Bruker - series Elexys E500 AR continuos wave, X Band (9GHz) with cavity Bruker ER4122SHQE, equipped with temperature control system Oxford ESR900 from 4 K to 350 K. It is also provided with cavity model ER4119HS-2100 with optical windows. This instrument is applied, together with the other EPR equipments, for the study of structure-activity relationships in metalloproteins, radical species, transition metal complexes via Multifrequency EPR spectroscopy. Furthermore, the Site-Directed Spin Labeling (SDSL) –EPR technique is used to study the interaction between bioactive peptides and biological EPR Bruker - series Elexys E500 AR continuos wave, X Band (9GHz) with cavity Bruker ER4122SHQE, equipped with temperature control system Oxford ESR900 from 4 K to 350 K. It is also provided with cavity model ER4119HS-2100 with optical windows. This instrument is applied, together with the other EPR equipments, for the study of structure-activity relationships in metalloproteins, radical species, transition metal complexes via Multifrequency EPR Bruker - series Elexys E500 AR continuos wave, X Band (9GHz) with cavity Bruker ER4122SHQE, equipped with temperature control system Oxford ESR900 from 4 K to 350 K. It is also provided with cavity model ER4119HS-2100 with optical windows. This instrument is applied, together with the other EPR equipments, for the study of structure-activity relationships in metalloproteins, radical species, transition metal complexes via Multifrequency EPR spectroscopy. Furthermore, the Site-Directed Spin Labeling (SDSL) –EPR technique is used to study the interaction between bioactive peptides and biological membranes.
  • Bruker ER200 D. Electron Paramagnetic Resonance. EPR Bruker ER200 D continuos wave equipped with a Jagmar-Poland S band (2-4GHz) microwave bridge and series of cavities Loop-Gap Resonators at 2.5 and at 4 GHz. This instrument is applied, together with the other EPR equipments, for the study of structure-activity relationships in metalloproteins, radical species, transition metal complexes via Multifrequency EPR spectroscopy. Furthermore, the Site-Directed Spin Labeling (SDSL) –EPR technique is used to study the interaction between bioactive peptides and biological membranes.
  • Varian E-112 Q. Electron Paramagnetic Resonance. Spectrometer Varian E-112 Q EPR system continuos wave, Varian E-110 Q Band (35GHz) Microwave Bridge and temperature control system from 77 K to room temperature. This instrument is applied, together with the other EPR equipments, for the study of structure-activity relationships in metalloproteins, radical species, transition metal complexes via Multifrequency EPR spectroscopy. Furthermore, the Site-Directed Spin Labeling (SDSL) –EPR technique is used to study the interaction between bioactive peptides and biological membranes.
  • BioLogic SFM-20. Freeze-Quench method Freeze-Quench technique to study and characterize transient radical species in enzymatic systems.
  • Jagmar-Poland Loop-Gap Resonators X Band. Electron Paramagnetic Resonance. This tool has high sensitivity for sample with high content of water.
  • IBM, HP and E4 Computer cluster with 12 nodes: HP-rx2600, IBM-xSeries 382 Itenium 2 and Intel Xeon (Woodcrest and Clovertown). High performance multiprocessors cluster to perform combined QM/MM and SDSL–EPR studies on conformationally photomodulable cyclic oligopeptides and DFT/PCM calculations on radical species and metal complexes.
  • JASCO FP-6500. Spectrofluorimetry. The FP-6500 is a research-grade spectrofluorometer with a minimum 1 nm resolution and a highest sensitivity.
  • Hewlett Packard HP8453 Diode Array UV/VIS spectrophotometer.

Up

Siena STCDB

  • TA Instruments Q1000. Differential Scanning Calorimetry.
  • Carlo Erba Instruments GC MEGA Series HRGC 5300. Gas Chromatography with Flame Ionization Detector (GC-FID). Separation technique for volatiles compounds between 30 and 300°C. Environmental analysis: separation and quantitation of pesticides and chlorinated compounds by a Alltech column “Pesticide 13662”.Analysis of volatiles with biological or toxicological importance, Amino-acids, Lipids, Alcohols, etc., by a column SUPELCO “SPB-5”.
  • Ion chromatograph DIONEX ICS3000, equipped with a conductometric, electrochemical and Photodiode Array detector (DIONEX PDA-1), and with an automatic sampler AS40. Quantitative determination of anions (chloride, bromide, phosphate, sulfate, iodide, etc.) and cations of metal ions and organic ions in different environmental and biological matrices.
  • Olympus IX50. Optical Microscopy. This inverted microscope is useful for many routine and research applications including advanced fluorescence techniques. Ocular 10x and magnification 4x, 10x, 20x.
  • Cambell Scientific CR10. Hydro-Meteorological Measurements (H-M). The hydro meteorological station able to measure: wind velocity and direction, atmospheric pressure, air humidity, air temperature. The UV-B, UV-A, PAR, Visible and global radiation are also measured. Water temperature (3 sensors), dissolved oxygen (2 sensors), pH (2 sensors)and Redox potential (2 sensors) are also measured.
  • Hydrolab Corporation DataSonde 3. Water Chemical Physical measurements (WCPM) .This unit able to measure temperature, pH, dissolved oxygen, conductivity (or salinity), turbidity and depth along the water column.
  • TA Instruments AR2000. The instrument suitable for the study of viscoelasticity, pouring point, yield stress and tixotropic properties of semisolid materials. Advanced controlled stress, direct strain and controlled rate. Twisting range: 0.0001-200 mN.m, resolution: 0.04 µrad, Normal Force control system: 0.01-50 N, angular velocity: max 300 rad/s. Environmental test chamber: heating rate max 60°C/min, temperature range between -160°C and 600°C
  • Perkin Elmer AAnalyst 100, HGA 800. Atomic Absorption Spectroscopy. The instrument allows the quantitative determination of V, Cr, Mn, Fe, Co, Ni, Cu, Ag, Cd, Pb but also As concentrations in water samples (superficial waters, water that were subjected to osmosis, sewage), soil, sediments, vegetable samples after suitable treatment. It is possible to determine also Ca, Mg, Na, K in the same samples using flame atomisation mode.
  • Biospherical Instruments CA (San Diego) PUV 541. Environmental Spectroscopy and ultraviolet and visible Spectral Irradiance measurements in water-Photochemistry and Photobiology (ESG UV-VisSIM). The units able to measure the spectral irradiance at 305, 313, 320 and 340 nm with spectral resolution of 1 nm. The photosynthetic active radiation is measured. Temperature, internal temperature and depth are also measured. The instrument can be released until 100 m below water surface.
  • Skye Instruments LTD (UK) Four Channels Light Sensor with Integral Amplifier SKR 1850A. Environmental Spectroscopy and Visible Spectral Irradiance measurements in water and air- Photochemistry and Photobiology ((ESG&VisSIM) P&P). This unit enables to measure radiation at 381, 441, 589 and 681 nm with a bandwidth of 10 nm. The unit can be used in the water until 5 m below water surface. Three identical units are present to measure the albedo. The visible channels matching most satellite platforms. These units can be used to validate the spectral irradiance measured by most popular satellite platforms.
  • StellarNet Inc. (Tampa, FL) EPP2000. Environmental Spectroscopy and Visible Spectral Irradiance measurements in water and air- Photochemistry and Photobiologyv((ESG&VisSIM) P&P).vThis unit able to measure the in air or underwater spectral irradiance between 280-851 nm with spectral resolution of 3 nm. This unit can be used for transmittance and absorbance measurements also.
  • Turner Design SCUFA II. Environmental Spectroscopy Photochemistry and Photobiology (ES P&P). This unit can measure the natural fluorescence and turbidity along the water column.
  • Solar Light Corp. UV-Biometer Model 501. Environmental Spectroscopy Photochemistry and Photobiology (ES P&P). This unit measure the damage of UV-B radiation based on erithemal action spectra. This unit can be deployed until 5 m below the water column.
  • Enclosures. Photochemistry and Photobiology (P&P). These are two enclosures (6000 L). These are used to gain information about the effect of different irradiance regimes on biotic and abiotic community in the shallow aquatic ecosystems.
  • Silicon Graphs workstation Octane. Environmental Spectroscopy and environmental studies (ES). This instrument is used with specific analysis programmes for the analysis of emitted or reflected radiation from ground sources, and measured by satellite sensors.
  • Physical Electronics TRIFT III. Secondary Ion Mass Spectrometry with Time of Flight Analyzer (ToF-SIMS). Sub-micron elemental, chemical and molecular characterization and imaging of solid surfaces and thin films. Time-of-Flight SIMS can analyze the outermost one or two mono-layers of a sample without etching the surface away. It is ideal for organic or inorganic materials, and can be used to characterize both insulating and conductive samples. With detection limits in the ppm to ppb range, shallow depth profiling capabilities and automated analysis, can be used to analyze surface contamination, trace impurities, thin films and it is also a valuable tool to investigate surface modification chemistry and catalyst surface composition.
  • Varian XL200MHz. Varian Gemini 200 MHz (4.75T) is equipped with 5 mm 1H/13C probe for acquisition of 1D and 2D spectra. The instrument can perform measures for the study of the structure and dynamics of biomolecules, as well as in vivo measurements of cell metabolism.
  • Varian VXR 300MHz. Varian Gemini 300 MHz is equipped with 5 mm 1H/13C broadband probe for acquisition of 1D and 2D spectra. The instrument can perform measures for the study of the structure and dynamics of biomolecules, as well as in vivo measurements of cell metabolism.
  • Bruker AMX-400MHz. Bruker AMX-400MHz is equipped with 5 mm 1H/13C broadband probe for acquisition of 1D and 2D spectra. The instrument can perform measures for the study of the structure and dynamics of biomolecules, as well as in vivo measurements of cell metabolism.
  • Bruker AVANCE DRX-600. AVANCE DRX-600, operating at 600 MHz for 1H (14T static magnetic field), is one of the most recent systems in NMR Spectroscopy. This is an instrument of new conception in which all the electronics, comprised the of acquisition, is digital . It is equipped with an ""Ultrashielded"" Magnex magnet, three channels for acquisition, gradients on X, Y, Z axis (50 gauss/cm) and an host computer Silicon Graphics O2 .The instrument is equipped with the following probes: QNP 10 mm with automatic software nucleus selection (1H/13C/23Na/31P); TBI 5 mm (1H/13C/31P/109Ag) with X, Y, Z gradients; BBO 10 mm 39K-41K;BBO 5 mm 31P-15N with Z gradient. The DRX 600 can carry out measures for the study of the structure and dynamics of biomolecules (from small molecules to proteins and nucleic acids) through the acquisition and analysis of 1D and 2D spectra and nuclear spin relaxation times, as well as in vivo measurements of cell metabolism.
  • Stelar Spin Master FFC2000 1Tesla. Fast Field Cycling Relaxometry. Fast Field Cycling Relaxometry (FFCR) is a NMR technique used to determine the longitudinal relaxation time (T1) over a range of magnetic fields spanning about 6 decades, from about 10-6 up to ~1 Tesla. This enormous range should be compared with the 0.1 T-20T interval currently covered by standard NMR superconducting magnets and variable-field electromagnets. This NMR technique can be used for studies in the following fields: paramagnetics: FFC relaxometry is the basic tool for studying the hydration of paramagnetic metal ions and their organometallic complexes in water solutions. Benefits include the development of contrast agents for clinical MR Imaging; Biochemistry: the function of proteins and other natural macromolecules present in cells and their membranes is often critically dependent upon their dynamics. Data on protein mobility are also useful to corroborate studies of their stereochemical structure; Liquid crystals: the complex molecular dynamics in liquid crystals is intimately related to the macroscopic characteristics of their various phases. FFC relaxometry has proved to a source of valuable data in this field; Polymers: molecular motions in bulk polymers have a profound effect on their properties. Physical properties of elastomers, for example, cannot be understood at all without a knowledge of their molecular mobility. Here FFC relaxometry will complement traditional techniques such as mechanical, dielectric and ultrasound techniques; Materials research: there are numerous classes of technological materials whose properties depend intimately on molecular dynamics (consider, for example, emulsions, lubricants, coatings, etc); Surface phenomena: molecular mobility at solid-liquid interfaces is important in catalysis, petrology and other fields (for example, relaxation data are already used to assay the distribution of pore sizes in rocks). FFC NMR shall introduce a new dimension in this field.
  • Amersham Biosciences Ultrospec 2000. UV/Visibile Spectrophotometry. Molecular Absorption Spectroscopy from 190 to 1100 nm wavelength. Water Quality and environmental analysis: Quantitative analysis of anions. Nucleic Acids analysis with a Peltier cell Holder: Nucleic Acids Quantification and Purity Checking, Protein Concentration on Nucleic Acids.
  • XRF Olympus Innov-X Delta plus equipped with Test Chamber and software for remote control. Portable instrument for the determination of elements (Mg, Al, Si, P, S, Cl, K, Ca, Ti Mn and other transition metals) in solid matrices. Measurements can be carried out on soil, minerals and metal alloys.
  • Lipex Biomembranes. Milestone ETHOS D. Microwave Labstation used for the mineralization of complex matrices. The treatment is executed on those samples which must then be analyzed through AAS.
  • FKV AMA 254. Automatic Solid/Liquid Hg Analyzer. The instrument concurs to determine mercury in solid and liquid samples without having to carry out a preliminary treatment of the sample. The sensibility is 0.1 ppb. Hg Analyzer

 

Up

Venice

  • Oxford Instruments, ISIS System Series 300. EDAX
  • SEM Jeol JSN-5600LV.
  • TEM Jeol JEM3010.LaB6 source; point-point res. 0.17 nm.
  • SAXS Anton Paar Kratky Compact.
  • WAXS Philips PW3020.
  • WAXS Philips PW1050.
  • Nicolet FT-IR NEXUS CONTINUUM Spectophotometer.  Accessories: diffuse reflectance (DRIFT). High pressure and high vacuum chambers, temperature up to 1173 K. HATR chamber. Resolution 0.1 cm-1, complete spectral range between 7200 and 50 cm-1.
  • Micromeritics Autochem 2910 (Automated Catalyst Characterization System). Instrument for chemisorption measurements. Controlled temperature desorption. Controlled temperature reduction TPR. Controlled temperature oxidation TPO.
  • Physisorpion Micromeritics.
  • Optical Metallography. Leica DMLM.


Up

[ Back ]

 
Copyright © CSGI 2012 - Credits - Cookie Policy - Privacy - Consorzio per lo Sviluppo dei Sistemi a Grande Interfase, via della Lastruccia 3, 50019, Sesto Fiorentino, Italy