logotype

PGOODs: new plasmids for the co-expression of proteins in Escherichia coli

Research Area: Uncategorized Year: 2011
Type of Publication: Article
Authors:
Journal: BIOTECHNOLOGY LETTERS Volume: 33
Number: 9 Pages: 1815-1821
Month: September
ISSN: 0141-5492
Abstract:
Two systems for the co-expression of proteins in Escherichia coli were designed and constructed. The first system relies on the new vector, pGOOD, which is compatible with ColE1-type plasmids and sustains efficient co-expression of soluble protein complexes. The second system is based on the pGOOD1 vector (a derivative of pGOOD), useful for the production of toxic proteins, whose synthesis can be regulated by the co-expressed LacI repressor.
[ Back ]
Copyright © CSGI 2012 - Credits - Cookie Policy - Privacy - Consorzio per lo Sviluppo dei Sistemi a Grande Interfase, via della Lastruccia 3, 50019, Sesto Fiorentino, Italy