Position: Permanent Staff
Phone: 051 2093-718
Over-expression of recombinant proteins in Escherichia coli.
Purification and characterization of the diphtheria toxin variant CRM197.
Bacterial replication and DNA Polymerase III.
Antisense RNAs and bacterial silencing.
Plant protein hydrolysates.
July 1998: Master degree in Applied Genetics, University of Bologna, Italy (70 cum laude/70). Title: “Phosphoprotein 85 kDa (pp85) of human Herpesvirus 7 (HHV-7)”.
1998-1999: CSGI fellowship at the University of Bologna on “Development of water-in-oil macroemulsions sustain long-term viability of microbial cells”.
1995-2005: Studies and research activity at: Virology and Microbiology Laboratory, Dept. of Experimental Pathology (University of Bologna); Marjorie B. Kovler Viral Oncology Laboratories” (University of Chicago, Illinois, USA); Plant Molecular Biology Laboratory, IMBB (Heraklion, Crete, Greece).
2005: PhD in Applied Biocatalysis and Industrial Microbiology at the University of Bologna. Title: "Structural analysis of antisense RNAs involved in gene silencing in Escherichia coli". Advisor: Prof. A. Hochkoeppler (University of Bologna).
2006-present: Researcher at the Department of Industrial Chemistry and Materials of the University of Bologna.
ASFV DNA polymerase extends recessed DNAs with catalytic efficiencies outperforming those exerted on gapped DNA substrates- Lapenna A. - Alessandra Stefan - Alejandro Hochkoeppler
Use of cotton textiles coated by ir(III) tetrazole complexes within ceramic silica nanophases for photo-induced self-marker and antibacterial application- Zanoni I. - Blosi M. - Fiorini V. - Crosera M. - Ortelli S. - Stagni S. - Alessandra Stefan - Psilodimitrakopoulos S. - Stratakis E. - Filon F.L. - Costa A.L.
Synthesis and Photochemical Properties of Manganese(I) Tricarbonyl Diimine Complexes Bound to Tetrazolato Ligands- Stout M.J. - Alessandra Stefan - Skelton B.W. - Sobolev A.N. - Massi M. - Alejandro Hochkoeppler - Stagni S. - Simpson P.V.
Substrate Activation of the Low-Molecular Weight Protein Tyrosine Phosphatase from Mycobacterium tuberculosis- Alessandra Stefan - Dal Piaz F. - Girella A. - Alejandro Hochkoeppler
Antibacterial activity of a new class of tris homoleptic Ru (II)-complexes with alkyl-tetrazoles as diimine-type ligands- Monti N. - Zacchini S. - Massi M. - Alejandro Hochkoeppler - Giorgini L. - Fiorini V. - Alessandra Stefan - Stagni S.
Purification from Deinococcus radiodurans of a 66 kDa ABC transporter acting on peptides containing at least 3 amino acids- Alessandra Stefan - Gentilucci L. - Piaz F.D. - D'Alessio F. - Santino F. - Alejandro Hochkoeppler
Structural and catalytic insights into HoLaMa, a derivative of Klenow DNA polymerase lacking the proofreading domain- Kovermann M. - Alessandra Stefan - Castaldo A. - Caramia S. - Alejandro Hochkoeppler
Luminescent protein staining with Re(i) tetrazolato complexes- Fiorini V. - Bergamini L. - Monti N. - Zacchini S. - Plush S.E. - Massi M. - Alejandro Hochkoeppler - Alessandra Stefan - Stagni S.
Purification of active recombinant human histone deacetylase 1 (HDAC1) overexpressed in Escherichia coli- Alessandra Stefan - Calonghi N. - Schipani F. - Dal Piaz F. - Sartor G. - Alejandro Hochkoeppler
Manganese is a deinococcus radiodurans growth limiting factor in rich culture medium- Borsetti F. - Dal Piaz F. - D’Alessio F. - Alessandra Stefan - Brandimarti R. - Sarkar A. - Datta A. - Silva A.M. - Den Blaauwen T. - Alberto M. - Spisni E. - Alejandro Hochkoeppler