The catalytic action of human D-lactate dehydrogenase is severely inhibited by oxalate and is impaired by mutations triggering D-lactate acidosis

The four subunits of rabbit skeletal muscle lactate dehydrogenase do not exert their catalytic action additively

The Mycobacterium tuberculosis protein tyrosine phosphatase MptpA features a pH dependent activity overlapping the bacterium sensitivity to acidic conditions

Trehalose counteracts the dissociation of tetrameric rabbit lactate dehydrogenase induced by acidic pH conditions

Amberlite XAD-4 is a convenient tool for removing Triton X-100 and Sarkosyl from protein solutions

High-yield production in Escherichia coli and convenient purification of a candidate vaccine against SARS-CoV-2

Allosteric transitions of rabbit skeletal muscle lactate dehydrogenase induced by pH-dependent dissociation of the tetrameric enzyme

Human lactate dehydrogenase A undergoes allosteric transitions under pH conditions inducing the dissociation of the tetrameric enzyme

ASFV DNA polymerase extends recessed DNAs with catalytic efficiencies outperforming those exerted on gapped DNA substrates

Production in Escherichia coli of recombinant COVID-19 spike protein fragments fused to CRM197

Synthesis and Photochemical Properties of Manganese(I) Tricarbonyl Diimine Complexes Bound to Tetrazolato Ligands

Substrate Activation of the Low-Molecular Weight Protein Tyrosine Phosphatase from Mycobacterium tuberculosis

Antibacterial activity of a new class of tris homoleptic Ru (II)-complexes with alkyl-tetrazoles as diimine-type ligands

Purification from Deinococcus radiodurans of a 66 kDa ABC transporter acting on peptides containing at least 3 amino acids

Structural and catalytic insights into HoLaMa, a derivative of Klenow DNA polymerase lacking the proofreading domain

Luminescent protein staining with Re(i) tetrazolato complexes

Purification of active recombinant human histone deacetylase 1 (HDAC1) overexpressed in Escherichia coli

Manganese is a deinococcus radiodurans growth limiting factor in rich culture medium

A single-molecule FRET sensor for monitoring DNA synthesis in real time

Methylation of Ir(III)-tetrazolato complexes: An effective route to modulate the emission outputs and to switch to antimicrobial properties

Dual role of imidazole as activator/inhibitor of sweet almond (Prunus dulcis) β-glucosidase

The thumb domain is not essential for the catalytic action of HoLaMa DNA polymerase

The expression of the Cuphea palustris thioesterase CpFatB2 in Yarrowia lipolytica triggers oleic acid accumulation

A continuous spectrophotometric enzyme-coupled assay for deoxynucleoside triphosphate triphosphohydrolases

Escherichia coli DnaE polymerase couples pyrophosphatase activity to DNA replication

The DnaE polymerase from Deinococcus radiodurans features RecA-dependent DNA polymerase activity

Structure of Escherichia coli dGTP Triphosphohydrolase: A hexameric enzyme with DNA effector molecules

The multifaceted benefits of protein co-expression in Escherichia coli

Simultaneous ternary extension of DNA catalyzed by a trimeric replicase assembled in vivo

HoLaMa: A Klenow sub-fragment lacking the 3′-5′ exonuclease domain

Expanding the landscape of recombinant protein production in Escherichia coli

A synthetic Post-transcriptional controller to explore the modular design of gene circuits

Stabilization of the Escherichia coli DNA polymerase III ε subunit by the θ subunit favors in vivo assembly of the Pol III catalytic core

Continuous enzyme-coupled assay of phosphate- or pyrophosphate-releasing enzymes

On the antibacterial activity of roots of Capparis spinosa L.

pGOODs: New plasmids for the co-expression of proteins in Escherichia coli

Overexpression and purification of the recombinant diphtheria toxin variant CRM197 in Escherichia coli

Shine-Dalgarno sequence enhances the efficiency of lacZ repression by artificial anti-lac antisense RNAs in Escherichia coli

Expression and purification of the recombinant mustard trypsin inhibitor 2 (MTI2) in Escherichia coli

Proteolysis of the proofreading subunit controls the assembly of Escherichia coli DNA polymerase III catalytic core

The regulatory elements of araBAD operon, contrary to lac-based expression systems, afford hypersynthesis of murine, and human interferons in Escherichia coli

Selenite transiently represses transcription of photosynthesis-related genes in potato leaves

Tyrosine83 is essential for the activity of E. coli galactoside transacetylase

Efficient silencing of the gene coding for the ε subunit of DNA polymerase III in Escherichia coli is triggered by antisense RNAs featuring stability in vivo

RNA complementary to the 5′ UTR of mRNA triggers effective silencing in Saccharomyces cerevisiae

Use of Rhodotorula minuta live cells hosted in water-in-oil macroemulsion for biotrasformation reaction

Polyphenol oxidase expression in potato (Solanum tuberosum) tubers inhibited to sprouting by treatment with iodine atmosphere

Purification of a polyphenol oxidase isoform from potato (Solanum tuberosum) tubers

Water-in-oil macroemulsions sustain long-term viability of microbial cells in organic solvents

Silencing of the gene coding for the ε subunit of DNA polymerase III slows down the growth rate of Escherichia coli populations